Purification, Characterization, and Application of Alkaline Protease Enzyme from a Locally Isolated Bacillus cereus Strain

Among the microbial enzymes protease and amylase are most valuable which have been has diversified applications used extensively because of their capabilities in degradation organic wastes, application biofuels, agricultural, pharmaceuticals, chemical biotechnological industries. The aim current research work was purification, characterization alkaline proteases extracted from Bacillus cereus AUST-7. Various concentrations ammonium sulphate were applied for enzyme precipitation. Sephadex-G 100 FPLC system separation other proteins. SDS-PAGE to measure molecular weight required protease. Relative activities determined against different pH, temperature, incubation period activity. Stability temperature various metal ions inhibiter also studied. Purified on goat skin explore dehairing efficacy. A 6.5 purification fold 1163.50 U/mg specific activity obtained at 70% saturation 35. 91 8902 observed after separation. 35 kDa size exhibited SDS-PAGE. purified stable pH 10, 55 °C min period. found be 8–11, thermo-stability 50 phenyl methyl sulphonyl fluoride (PMSF) di-isopropyl fluorophosphates (DFP) inhibited good potential as agent leather